GLYCOSIDASE-CATALYZED OLIGOSACCHARIDE SYNTHESIS - PREPARATION OF THE N-ACETYLCHITOOLIGOSACCHARIDES PENTA-N-ACETYLCHITOPENTAOSE AND HEXA-N-ACETYLCHITOHEXAOSE USING THE BETA-N-ACETYLHEXOSAMINIDASE OF ASPERGILLUS-ORYZAE

Authors
SINGH S GALLAGHER R DERRICK PJ CROUT DHG
Citation
S. Singh et al., GLYCOSIDASE-CATALYZED OLIGOSACCHARIDE SYNTHESIS - PREPARATION OF THE N-ACETYLCHITOOLIGOSACCHARIDES PENTA-N-ACETYLCHITOPENTAOSE AND HEXA-N-ACETYLCHITOHEXAOSE USING THE BETA-N-ACETYLHEXOSAMINIDASE OF ASPERGILLUS-ORYZAE, Tetrahedron : asymmetry, 6(11), 1995, pp. 2803-2810
Citations number
24
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Inorganic & Nuclear","Chemistry Physical
Journal title
Tetrahedron : asymmetry → ACNP
ISSN journal
09574166
Volume
6
Issue
11
Year of publication
1995
Pages
2803 - 2810
Database
ISI
SICI code
0957-4166(1995)6:11<2803:GOS-PO>2.0.ZU;2-I
Abstract
Using a crude beta-N-acetylhexosaminidase from Aspergillus oryzae both tri-N-acetylchitotriose (GlcNAc)(3) (1, n=1) and tetra-N-acetylchitot etraose (GlcNAc)(4) (1, n=2) act respectively as both glycosyl donor a nd glycosyl acceptor to give product mixtures containing significant q uantities of the corresponding penta- and hexasaccharides [1 (n=3) and 1 (n=4), respectively] which are readily isolated and purified by cha rcoal-Celite chromatography.