Protective capacity of a IgM/IgA-enriched polyclonal immunoglobulin-G preparation in endotoxemia

Animal experiments were carried out to investigate whether a protective eff ect can be achieved in endotoxemia by intravenous (i.v.) application of a p olyclonal immunoglobulin preparation (IVIG-IgG/A/M) enriched with 12% IgM a nd 12% IEA. Following administration of IVIG-IgG/A/M (500 mg/kg), endotoxem ia was induced by intraperitoneal inoculation of a sublethal dose (5x10(8) CFU/kg) of Escherichia coli (E. coli) and subsequent i.v. administration of an antimicrobial agent (Imipenem). Plasma endotoxin activity, IL-6 activit y, mean arterial pressure, and skeletal muscle oxygen pressure (tpO(2)) wer e measured at regular intervals over a total observation period of 7 h. Pro phylactic administration of IVIG-IgG/A/M was found to significantly attenua te (P<0.01) the antibiotic-induced increase in endotoxin activity as compar ed to the albumin control group. Limited endotoxemia in the IgG/A/M group w as associated with reduced levels of circulating IL-6 (P<0.01). Both lipopo lysaccharide-induced hypotension and depression of tissue oxygenation were attenuated (P<0.01) by pre-treatment with IVIG-IgG/A/M. The experimental re sults suggest that in endotoxemia the polyclonal immunoglobulin preparation has a prophylactic protective effect on the acute phase responses and redu ces the cardiodepressant effects of E. coli septicaemia.