Solid phase synthesis of protected peptide nucleic acids

Boc/Z-protected PNA oligomers were synthesised on solid phase. The use of t he allylic HYCRON resin allowed for the application of both Boc- and Fmoc-p rotecting groups. Highest yields were obtained when the monomeric building block was synthesised on solid phase rather than loaded as preformed unit. The selective attachment of fluorescent labels at the C-terminal (3') end d emonstrated for the first time that further manipulations on protected PNA fragments are feasible. (C) 1999 Published by Elsevier Science Ltd. All rig hts reserved.