N. Haghighat et I. Al-hashimi, A pilot study on the effect of radiation on calmodulin in rat submandibular salivary glands, ARCH ORAL B, 44(5), 1999, pp. 383-389
Xerostomia and loss of salivary gland secretion is one of the most common c
omplications of the radiation treatment of head-and-neck malignancies. The
secretory mechanism in the salivary glands can be modulated by the concentr
ation of intracellular Ca2+. Calmodulin is a calcium-binding protein that i
s widely distributed in nature and is involved in regulating intracellular
calcium. In this study the effect of radiation on the concentration of calm
odulin in rat salivary glands was investigated. Fourteen rats were divided
into three groups: R1 (n = 4) and R2 (n = 5) received a single dose of 15 G
y and group C (n = 5) received no radiation. R1 and R2 animals were killed
at weeks 2 and 10 post-irradiation, respectively. The submandibular glands
were removed, homogenized and their total calmodulin was determined. The me
an calmodulin concentrations were 6.4+/-1.1 mu g/gland for controls, 14.1+/
-3.7 mu g/ gland for R1 and 68.2+/-14.4 mu g/gland for R2. Kruskal-Wallis A
NOVA revealed a significant increase in the concentration of calmodulin fol
lowing irradiation (p = 0.003). The relationship between this increase and
the loss of salivary gland function is not yet clear. (C) 1999 Elsevier Sci
ence Ltd. All rights reserved.