Genome structure and chromosomal mapping of the gene for Fe65L2 interacting with Alzheimer's beta-amyloid precursor protein

Recently we cloned the cDNA encoding human Fe65L2, which interacts with Alz heimer's beta-amyloid precursor protein (APP), The protein has one WW domai n and two PID elements (Neurosci. Lett. (1999) 261., 143-146.). Here, we re port the entire genome structure and the chromosomal mapping of the Fe65L2 gene. The gene is composed of thirteen coding exons distributed over 6 kb a nd the genomic organization is similar to another Fe65 member, Fe65 gene Tw o transcription start sites of the gene were found in a CpG island by prime r extension analysis. Radiation hybrid mapping revealed that the Fe65L2 gen e is on chromosome 5 between markers SHGC-9824 (D5S2374) and SHGC-8489 (D5S 2569), Characterization of the Fe65L2 gene structure will be useful in the linkage analysis and search for mutations in the Fe65L2 gene in patients wi th Alzheimer's disease. (C) 1999 Academic Press.