Nh. Jeoung et al., Identification of retinoic acid receptor element in human cholesteryl ester transfer protein gene, BIOC BIOP R, 258(2), 1999, pp. 411-415
Citations number
28
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
The transcriptional regulation of the human cholesteryl ester transfer prot
ein (CETP) gene by retinoic acid was investigated by a transient transfecti
on assay. A series of deleted vectors generated from the 5'-upstream region
(3434 bp) of the CETP gene linked to the luciferase reporter gene was indi
vidually transfected to HepG2 cells, Promoter analyses revealed essential r
egulatory machinery in the -110/-40 region of the upstream sequence of the
human CETP gene. When the cells transfected with the reporter vectors were
stimulated with all-trans retinoic acid (tRA), the hormone response was dra
stically reduced when the -165/-110 region was deleted, thereby suggesting
that there may be a retinoic acid receptor element (RARE) in the region. A
footprinting analysis showed that the DNA segment at the -165/-134 is prote
cted by the HepG2 nuclear extract. A competition analysis on the gel mobili
ty shift assay using consensus RARE and a purified retinoic acid receptor c
onfirmed the -165/-134 region as being RARE. (C) 1999 Academic Press.