S. Hashimoto et al., Thioredoxin negatively regulates p38 MAP kinase activation and IL-6 production by tumor necrosis factor-alpha, BIOC BIOP R, 258(2), 1999, pp. 443-447
Citations number
36
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
We examined the regulatory role of a reduction/oxidation (redox) control pr
otein, thioredoxin (TRX), in tumor necrosis factor-alpha (TNF-alpha)-induce
d p38 MAP kinase activation and p38 MAP kinase-mediated cytokine expression
utilizing TRX-transfected murine L929 cells (TRX14). The results showed th
at TNF-alpha-induced p38 MAP kinase activation and interleukin-6 (IL-6) pro
duction by TRX 14 were less than those by the parental L cells and the cont
rol transfected L cells (Neo-1). SB 203580 as the specific inhibitor for p3
8 MAP kinase activity inhibited TNF-alpha-induced IL-6 production by the pa
rental L cells, indicating that TNF-alpha-activated p38 MAP kinase regulate
s IL-6 production by the cell lines used in this study. These results showe
d that overexpression of TRX negatively regulates p38 MAP kinase activation
and p38 MAP kinase-mediated IL-6 production by TNF-alpha-stimulated cells,
indicating that TRX is critical for p38 MAP kinase activation which regula
tes cytokine expression. (C) 1999 Academic Press.