Theoretical aspects of single pulse induced modulation of cell-cell interactions favoring iso-electrofusion

A single pulse-mediated electrofusion of freely suspending widely separated cells could reduce steps in the protocol. Prediction of such electrofusion is still unsolved. In pre-pulse condition, quantitative estimation with th ree-layered model cell surface reveals electrostatic repulsion (due to nega tive surface charge) is the major hindrance to membrane-to-membrane contact . On theoretical grounds we predict that designed single high voltage pulsi ng on widely separated model cell surface can counteract the non-specific r epulsive interaction and favor approach of two apposed membranes. But hydro dynamic modulation of pulse exposed cell surface interaction can hamper app roach of membranes contact when cells are held at a gap of more than simila r to 450 Angstrom. (C) 1999 Elsevier Science S.A. All rights reserved.