Multiple-pulse-mediated electrofusion of intact erythrocyte onto human term placental amnion

The creation of surface modified human term placental amnion by electrofusi ng human cells onto its surface has been thought of. A multiple-pulse elect rofusion protocol with 10 square pulses of 10-mu s pulse length, and electr ic field of 0.2 kV cm(-1), can make erythrocyte-amnion tissue electrofusion possible. The protocol devised merge the cell-tissue-adherence steps with fusogenic pulse. The finding opens up a new avenue of cell electrofusion on to human tissue with minimal procedural complexities. (C) 1999 Elsevier Sci ence S.A. All rights reserved.