Defined starter systems, consisting of bacteriocin-tolerant Lactococcus lac
tis subsp. lactis H6 alone or in combination with bacteriocin-sensitive L.
lactis subsp. cremoris H1, and low amounts of a bacteriocin-producing cultu
re, were developed and used for the manufacture of semi-hard cheese. Aminop
eptidase activity and proteolysis were increased and acidification retarded
in cheeses made from milk inoculated with lactococci and the bacteriocin-p
roducing culture with respect to cheeses from milk inoculated with only lac
tococci. Cheeses made with a defined-strain starter system consisting of L.
lactis subsp. lactis H6 and the bacteriocin-producing culture received the
highest scores for flavour intensity and quality.