H. Inohara et al., Expression of galectin-3 in fine-needle aspirates as a diagnostic marker differentiating benign from malignant thyroid neoplasms, CANCER, 85(11), 1999, pp. 2475-2484
BACKGROUND. Galectin-3 is a beta-galactoside-binding protein that has been
reported to be expressed preferentially in thyroid malignancies. The curren
t study was designed to substantiate this finding further and to establish
a presurgical diagnostic modality of differentiating between benign and mal
ignant thyroid neoplasms by analyzing galectin-3 expression in fine-needle
aspirates.
METHODS. The expression of galectin-3 was examined immunohistochemically in
total of 172 specimens: 45 primary and 20 metastatic papillary carcinomas,
8 primary and 2 metastatic follicular carcinomas, 5 primary and 3 metastat
ic anaplastic carcinomas, 3 primary medullary carcinomas, 25 follicular ade
nomas, 3 goiters, and 58 adjacent normal thyroid tissue. Alternatively, epi
thelial cells were isolated from the fine- needle aspirates of 14 thyroid n
odules and subjected to immunoblotting analysis of galectin-3.
RESULTS. Immunohistochemical analysis revealed that all thyroid malignancie
s of follicular cell origin (including papillary, follicular, and anaplasti
c carcinomas) showed high and diffuse expression of galectin-3, whereas one
of the three medullary carcinomas of parafollicular cell origin displayed
weaker and focal expression of galectin-3. In contrast, neither benign thyr
oid adenomas, goiters, nor normal thyroid tissues expressed galectin-3. Imm
unoblot analysis of the isolated epithelial cells detected galectin-3 in ni
ne thyroid nodules that were proven histologically to be malignant (eight p
apillary carcinomas and one follicular carcinoma) after surgical interventi
on, whereas galectin-3 was not detected in five nodules proven to be benign
follicular adenomas.
CONCLUSIONS. Galectin-3 serves as a marker of thyroid malignancy of follicu
lar cell origin. Analysis of galectin-3 expression in fine-needle aspirates
enhances the differential diagnostic accuracy between benign and malignant
thyroid neoplasms. Cancer 1999;85:2475-84, (C) 1999 American Cancer Societ
y.