M. Cay et M. Naziroglu, Effects of intraperitoneally-administered vitamin E and selenium on the blood biochemical and haematological parameters in rats, CELL BIOC F, 17(2), 1999, pp. 143-148
The aim of this work was to determine the role of intraperitoneally-adminis
tered vitamin E and selenium on the biochemical and haematological paramete
rs in the blood of rats. Thirty-two adult male Wistar rats were used in thi
s study. All rats were randomly divided into four groups. The first group w
as used as the control. The second group was intraperitoneally administered
with vitamin E (+/-alpha-tocopheroryl acetate, 10 mg day(-1)), the third g
roup with Se (Na2SeO3 0.2 mg over a day), and the fourth group with vitamin
E and Se (vitamin E 10 mg + Na2SeO3 0.2 mg over a day). This administratio
n was done for 5 weeks. Blood samples were taken from animals at the end of
the dosage period and biochemical parameters in serum samples and haematol
ogical parameters in total blood were determined. The levels of total chole
sterol (p < 0.01) and number of white blood cells (p < 0.001) in blood were
significantly higher in the vitamin E group than in the control group. The
levels of ALP, total cholesterol (p < 0.01) and number of white blood cell
s(p < 0.01) in blood were significantly higher in the selenium group than i
n the controls. The levels of glucose (p < 0.05), ALP (p < 0.01), total cho
lesterol (p < 0.001) and number of white blood cells (p < 001) were higher
in the vitamin E and selenium combined group than in the controls. Other pa
rameters considered within this trial (ALT, LDH, creatinine, albumin, total
protein, amylase, creatine kinase, HDL, triglycerides, total lipid, sodium
, chloride, uric acids, red blood cell, haemoglobin, packed cell volume, MC
V, MCH, MCHC) did not show statistically significant differences between th
e control and injected groups. The results indicated that blood glucose and
total cholesterol levels, ALP activity and white blood cell counts were si
gnificantly increased by intraperitoneal administration of vitamin E and se
lenium in rats. Copyright (C) 1999 John Wiley & Sons, Ltd.