Detection of ileS-2 gene encoding mupirocin resistance in methicillin-resistant Staphylococcus aureus by multiplex PCR

Authors
Nunes, ELD dos Santos, KRN Mondino, PJJ Bastos, MDD Giambiagi-deMarval, M
Citation
Eld. Nunes et al., Detection of ileS-2 gene encoding mupirocin resistance in methicillin-resistant Staphylococcus aureus by multiplex PCR, DIAG MICR I, 34(2), 1999, pp. 77-81
Citations number
21
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
ISSN journal
07328893 → ACNP
Volume
34
Issue
2
Year of publication
1999
Pages
77 - 81
Database
ISI
SICI code
0732-8893(199906)34:2<77:DOIGEM>2.0.ZU;2-V
Abstract
The presence of the ileS-2 gene, responsible for mupirocin resistance, in c linical isolates of methicillin-resistant Staphylococcus aureus was determi ned by multiplex polymerase chain reaction. Three pairs of primers were use d, which yielded specific fragments of femA (encoding a unique feature of S . aureus), mecA (encoding resistance to methicillin) and ileS-2 genes. The multiplex polymerase chain reaction system is an easy and time-saving techn ique that, together with a rapid method for DNA extraction by boiling, may be incorporated as a routine analysis in clinical diagnostic laboratories. (C) 1999 Elsevier Science Inc.