Eld. Nunes et al., Detection of ileS-2 gene encoding mupirocin resistance in methicillin-resistant Staphylococcus aureus by multiplex PCR, DIAG MICR I, 34(2), 1999, pp. 77-81
The presence of the ileS-2 gene, responsible for mupirocin resistance, in c
linical isolates of methicillin-resistant Staphylococcus aureus was determi
ned by multiplex polymerase chain reaction. Three pairs of primers were use
d, which yielded specific fragments of femA (encoding a unique feature of S
. aureus), mecA (encoding resistance to methicillin) and ileS-2 genes. The
multiplex polymerase chain reaction system is an easy and time-saving techn
ique that, together with a rapid method for DNA extraction by boiling, may
be incorporated as a routine analysis in clinical diagnostic laboratories.
(C) 1999 Elsevier Science Inc.