Structure of VAT, a CDC48/p97 ATPase homologue from the archaeon Thermoplasma acidophilum as studied by electron tomography

Valosine-containing protein-like ATPase from Thermoplasma acidophilum is a member of the superfamily of ATPases associated with a diversity of cellula r activities and is closely related to CDC48 from yeast and p97 from higher eukaryotes and more distantly to N-ethylmaleimide-sensitive fusion protein . We have used electron tomography to obtain low-resolution (2-2.5 nm) thre e-dimensional maps of both the whole 500 kDa complex and the N-terminally t runcated valosine-containing protein-like ATPase from T. acidophilum comple x lacking the putative substrate binding domain. (C) 1999 Federation of Eur opean Biochemical Societies.