Identification and characterization of G90, a novel mouse RNA that lacks an extensive open reading frame

We describe the cloning and characterization of the murine G90 gene, identi fied by subtractive hybridization based on the differential presence of its transcript in large and small intestine. The full-length cDNA and genomic sequences were cloned and found to produce a 1.5 kb transcript that is poly adenylated but has no open reading frame larger than 249 bp. The G90 gene w as mapped to the proximal region of mouse chromosome 6. Expression analysis by Northern blotting showed that G90 is transcribed at very high levels in the small intestine and at lower levels an large intestine, testis and kid ney of the mouse. In situ hybridization analysis on sections of small and l arge intestine and testis showed that G90 transcripts are present only in p ost-mitotic cells. (C) 1999 Elsevier Science B.V. All rights reserved.