A. Hiasa et al., Retroviruses prepared from human DAF expressing murine packaging cells acquire resistance against human serum, INT J ONCOL, 14(6), 1999, pp. 1091-1096
The complement system of the human body inactivates the infectious ability
of retroviruses injected as an artificial gene transfer vector. We establis
hed new murine leukemia virus (MuLV) packaging cell lines; D2SS and D7S whi
ch express decay-accelerating factor (DAF) on their surface. Both D2SS and
D7S were resistant against incubation with fresh human serum. Moreover, the
retroviruses produced from these packaging cell lines were also resistant
to serum treatment. This resistance can be inhibited by DAF neutralizing an
tibody 1C6. These data demostrate that DAF induces a partial protection of
MuLV infection from the human complement system.