Growth of HPV-18 immortalized human prostatic intraepithelial neoplasia cell lines. Influence of IL-10, follistatin, activin-A, and DHT

Cultures from high grade prostatic intraepithelial neoplasia (HGPIN) have b een established and immortalized by HPV-18 infection. The cultures were ide ntified as PIN by Western blotting with anti-cytokeratin (34 beta E12) and prostate specific antigen (PSA) antibodies. We examined the growth capabili ties of the cultures in the presence of TGF-beta 1, activin-A, follistatin (FS), androgens (DHEA, DHT) and several cytokines (IL-10, IL-2, IL-4). IL-1 0, FS, and DHT stimulated cell proliferation and colony forming ability, wh ile the other cytokines and growth factors had no discernable effect. In ad dition, DHT and to a lesser extent IL-10 both stimulated PSA production. Ac tivin-A blocked IL-10, FS, and DHT stimulated growth and PSA production. We interpret the data to mean that IL-IO induction of FS secretion (and FS bi nding of activin A) restores the normal growth capabilities of HGPIN cultur es.