J. Onken et Rg. Berger, Effects of R-(+)-limonene on submerged cultures of the terpene transforming basidiomycete Pleurotus sapidus, J BIOTECH, 69(2-3), 1999, pp. 163-168
The biotransformation of limonene by the basidiomycete Pleurotus sapidus yi
elded cis/trans-carveol and carvone as the main products. The transformatio
n period was extended from 4 days after direct addition to 12 days by gas p
hase addition of the substrate. After 2 days of transformation, 97% of the
substrate had accumulated in the mycelium, while only 3% were present in th
e culture medium. Substrate toxicity led to a decrease of dry matter. Adapt
ation of the precultures with small amounts of substrate doubled the concen
tration of carveol and increased the concentration of carvone by a factor o
f 3-4. Total product concentrations of > 100 mg l(-1) were reached. (C) 199
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