The advent of new technology and the unmet needs of old and new epidemics o
f infectious diseases have stimulated a new era of vaccinology. One of the
most novel approaches employs plasmid DNA engineered to express one or more
genes of the pathogen in mammalian cells. Plasmids may also express cytoki
ne or costimulatory molecules to 'direct' the immune response and/or expres
s altered forms of the antigen to direct it to a specific intracellular com
partment or a specific extracellular receptor. The quality of immune respon
ses generated by DNA vaccines in animals has previously only been equaled b
y live attenuated viral vaccines. The immune stimulating activity of DNA va
ccines, combined with their versatility, suggests vast potential for these
vaccines.