Citation
Hjcm. Sterenborg et al., A novel frequency domain fluorescence technique for determination of triplet decay times, PHYS MED BI, 44(5), 1999, pp. 1419-1426
Citations number
23
Categorie Soggetti
Multidisciplinary
Journal title
PHYSICS IN MEDICINE AND BIOLOGY
ISSN journal
00319155
→ ACNP
Volume
44
Issue
5
Year of publication
1999
Pages
1419 - 1426
Database
ISI
SICI code
0031-9155(199905)44:5<1419:ANFDFT>2.0.ZU;2-G
Abstract
Frequency domain fluorescence measurement using two diode lasers with ampli
tude modulation in the kHz range yields a signal component at the sum frequ
ency. This intermodulation phenomenon was observed in an aqueous solution o
f haematoporphyrin (HP) and could be related to triplet state population ki
netics. This indirect measurement technique may allow triplet decay time me
asurement during photodynamic therapy (PDT) enabling monitoring of the type
II phototoxic damage rate.