Regeneration of plantlets from cotyledon and embryonal axis explants of Vigna mungo L-Hepper

Authors
Ignacimuthu, S Franklin, G
Citation
S. Ignacimuthu et G. Franklin, Regeneration of plantlets from cotyledon and embryonal axis explants of Vigna mungo L-Hepper, PL CELL TIS, 55(1), 1998, pp. 75-78
Citations number
21
Categorie Soggetti
Plant Sciences
Journal title
PLANT CELL TISSUE AND ORGAN CULTURE
ISSN journal
01676857 → ACNP
Volume
55
Issue
1
Year of publication
1998
Pages
75 - 78
Database
ISI
SICI code
0167-6857(1998)55:1<75:ROPFCA>2.0.ZU;2-7
Abstract
Sexually mature black gram plantlets were efficiently regenerated from seed -derived cotyledon and embryonal axis explants.The explants were capable of producing multiple shoots when cultured on modified MS medium containing B 5 vitamins supplemented with 6-benzylaminopurine (BAP) and alpha-naphthalen eacetic acid (NAA) within 15 days. The regenerated shoots were elongated on MS basal medium and rooted on MS medium containing indole-3-butyric acid ( IBA). Rooted plantlets were transferred to soil with 75% success, where the y attained sexual maturity and produced viable seeds.