Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae (Reprinted from Proc. Natl. Acad. Sci, vol 68, pg 2913-2917, 1971)

A bacterial restriction endonuclease has been used to produce specific frag ments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 wit h the restriction endonuclease from Hemophilus influenzae gave II fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equi molar with the original DNA. The fragments ranged from about 6.6 x 10(5) to 7.4 x 10(4) daltons, as determined by electron microscopy, DNA content, or electrophoretic mobility.