Short communication: Acetonitrile-hydrolysing enzymes in Rhodococcus erythropolis A10

Authors
Acharya, A Desai, AJ
Citation
A. Acharya et Aj. Desai, Short communication: Acetonitrile-hydrolysing enzymes in Rhodococcus erythropolis A10, WORLD J MIC, 15(1), 1999, pp. 123-125
Citations number
7
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN journal
09593993 → ACNP
Volume
15
Issue
1
Year of publication
1999
Pages
123 - 125
Database
ISI
SICI code
0959-3993(199902)15:1<123:SCAEIR>2.0.ZU;2-O
Abstract
Rhodococcus erythropolis A10 metabolizes acetonitrile by a two step process involving nitrile hydratase (NHase) and amidase. Both the enzymes were ind ucible and low basal levels of activities were observed in the cells grown in the absence of acetonitrile (AN). Cobalt and iron enhanced NHase, while amidase showed iron dependence. Presence of glucose or ammonium sulphate (A S) failed to affect acetonitrile utilization.