In this review paper, the term 'availability' is used to describe the suppl
y of nutrients and energy from the digestive tract to the body tissues. The
traditional method for the estimation of nutrient and energy availability
is to determine the apparent digestibility. This method has been a cornerst
one in the development of all existing feed evaluation systems, both in rum
inant and in non-ruminant nutrition. The apparent digestibility of a nutrie
nt is an underestimation of the true digestibility to the extent that endog
enous secretions of this nutrient are excreted in the faeces. Other factors
such as microbial degradation and syntheses in the digestive tract contrib
ute to invalidate the apparent digestibility as a measure of nutrient avail
ability. These problems are particularly related to protein and amino acids
. Alternative methods to estimate nutrient availability have been developed
. Some of these are invasive and some are non-invasive. In the first catego
ry, pigs are cannulated at various sites of the digestive tract or catheter
ized in an artery and the portal vein. Non-invasive methods include isotopi
c labelling of the feed, in vitro assays and the use of mechanistic modelli
ng. The virtues and drawbacks of these methods are discussed and it is conc
luded that progress in the field of predicting nutrient availability and in
the development of future, nutrient-based feed evaluation systems is most
likely to occur when various experimental and modelling techniques are inte
grated.