PHARMACOKINETIC STUDY OF 3 SYNTHETIC AT-BINDING PENTASACCHARIDES IN VARIOUS ANIMAL SPECIES EXTRAPOLATION TO HUMANS

Citation
Jp. Herault et al., PHARMACOKINETIC STUDY OF 3 SYNTHETIC AT-BINDING PENTASACCHARIDES IN VARIOUS ANIMAL SPECIES EXTRAPOLATION TO HUMANS, Blood coagulation & fibrinolysis, 8(3), 1997, pp. 161-167
Citations number
18
Categorie Soggetti
Hematology
ISSN journal
09575235
Volume
8
Issue
3
Year of publication
1997
Pages
161 - 167
Database
ISI
SICI code
0957-5235(1997)8:3<161:PSO3SA>2.0.ZU;2-Q
Abstract
The 'synthetic pentasaccharide', SR 90107A/Org 31540 (SP) representing the minimal AT-binding sequence of heparin is a catalyst of factor Xa inhibition. Affinity of SP, Sanorg 32701 (32701) and SR 80027 (80027) , two close analogues of SP for rat, rabbit, baboon and human AT has b een evaluated. The dissociation constants (Kd) for AT of the three spe cies were in the range of 41-132, 1.4-6.2 and 2.8-4.6 nM for SP, 80027 and 32701, respectively. Comparative pharmacokinetics (PK) of their a nti-factor Xa activities were determined in rats, rabbits and baboons. An apparent correlation could be demonstrated between half-lives of e limination and the corresponding Kd for AT in rats and rabbits whereas in baboons, such a correlation was not found. These results showed th at despite Kd values ten times lower for 32701 than for SP, both compo unds showed close PK parameters in baboons whereas the very low Kd val ue for 80027 was associated with an extended terminal half-life in thi s species. The predicted human PK parameters were determined using an allometric model: an empirical approach based on the integration of da ta obtained in various animal species which allows the extrapolation t o humans. The values obtained for the terminal half-lives of SP, 80027 and 32701 were 14.1, 88 and 6.5 h, respectively. For SP, calculation by allometry correlated well with the values observed in man. Since kn owledge of duration of activity is pivotal for appropriate design of p hase I clinical studies of anti-Xa oligosaccharides, allometry appears to be an interesting tool to predict the duration of action of such c ompounds in man.