Use of a peroxidase reactor in flow injection analysis for the determination of chloramine and the inhibition kinetics

A method for the determination of chloramine using a flow injection peroxid ase reactor based on the inhibition reaction of the enzyme is developed. Th e immobilisation of the horseradish peroxidase is performed on the commerci al polymer carrier VA Epoxy Biosynth. The peroxidase activity is detected p hotometrically based on the dehydration of the dye 2,2'-azino-di-[3-ethylen ebenzthiazoline-6-sulphonate] (ABTS). The calibration of the method gives a linear concentration range from 0.026 to 1.04 mmol l(-1) (SDn=3=below 5%). The detection limit was calculated to 26 mu mol l(-1). A mixture out of co mpetitive and noncompetitive inhibition was analysed based on the Lineweave r-Burk plots. (C) 1999 Elsevier Science B.V. All rights reserved.