Continuous-flow and flow injection pH gradients for spectrophotometric determinations of mixtures of nucleic acid components

A procedure for the rapid determination of mixtures of nucleic acid compone nts from the analysis of spectrophotometric multivariate data obtained with continuous-flow and now injection pH-gradient systems is proposed. Three n ow systems have been developed and assayed in which an on-line pH gradient is generated from the mixing and controlled dispersion of acidic and basic titrant solutions. Quantitative determinations of any particular analyte in the unknown samples in the presence of interferences ig performed with a s ingle pure standard for this analyte. They are carried out using an alterna ting least squares multivariate curve resolution procedure. The methods pro posed have been validated using synthetic and real sample mixtures. The res ults obtained are concordant with the labeled values, and the relative pred iction errors are around 5%.