Jl. Mumford et al., Detection of p53 protein accumulation in sputum and lung adenocarcinoma associated with indoor exposure to unvented coal smoke in China, ANTICANC R, 19(2A), 1999, pp. 951-958
Lung cancer in Xuan Wei (XW), China has been linked to exposure to unvented
coal smoke and adenocarcinoma, especially bronchioloalveolar carcinoma, is
most common, p53 mutations occur commonly in lung cancers and usually gene
rate detectable levels of p53 protein accumulation. Sputum is noninvasive t
o collect and ideal for screening p53 abnormalities, p53 protein accumulati
on was detected by immunohistochemistry in lung tumors and sputa from XW lu
ng cancer patients to determine (1) the role of p53 in lung pathogenesis, a
nd (2) feasibility of detecting p53 protein accumulation in sputum. p53 pro
tein accumulation was detected in 73% (22/30) of lung adenocarcinomas from
XW females exposed to coal emissions and significantly higher than the cont
rol cases (33%, p<0.05). In sputum, we detected p53 overexpression in tumor
cells in 54% (13/24) of XW cases and also in dysplastic cells (50% or 4/8)
. These findings suggest that p53 abnormalities is important in XW lung can
cer etiology.