High-level production of heterologous protein by engineered yeasts grown in cottage cheese whey

Authors
Maullu, C Lampis, G Desogus, A Ingianni, A Rossolini, GM Pompei, R
Citation
C. Maullu et al., High-level production of heterologous protein by engineered yeasts grown in cottage cheese whey, APPL ENVIR, 65(6), 1999, pp. 2745-2747
Citations number
23
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
6
Year of publication
1999
Pages
2745 - 2747
Database
ISI
SICI code
0099-2240(199906)65:6<2745:HPOHPB>2.0.ZU;2-0
Abstract
Cottage cheese whey is a cheese industry by-product still rich in proteins and lactose. Its recycling is seldom cost-effective. In this work we show t hat the lactose-utilizing yeast Kluyveromyces lactis, engineered for produc tion of recombinant human lysozyme, can be grown in cottage cheese whey, re sulting in high-level production of the heterologous protein (125 mu g/ml).