Virus passage through track-etch membranes modified by salinity and a nonionic surfactant

Citation
Cd. Lytle et al., Virus passage through track-etch membranes modified by salinity and a nonionic surfactant, APPL ENVIR, 65(6), 1999, pp. 2773-2775
Citations number
13
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
6
Year of publication
1999
Pages
2773 - 2775
Database
ISI
SICI code
0099-2240(199906)65:6<2773:VPTTMM>2.0.ZU;2-J
Abstract
Why do viruses sometimes not pass through larger pores in track-etch filter s? Increasing the salinity (0.8 to 160 mM Na+) decreased phi X174 and PRD1 passage through track-etch polycarbonate membranes (sodium dodecyl sulfate coated but not polyvinylpyrrolidone coated) and PRD1 passage through polyes ter membranes. Undiminished passage when 0.1% Tween 80 was added implied th at nonionic virus adsorption occurred and indicated that high levels of sal inity decreased virus passage by decreasing electrostatic repulsion that pr evented adsorption.