Determination of 5 '-leader sequences from radically disparate strains of porcine reproductive and respiratory syndrome virus reveals the presence ofhighly conserved sequence motifs
Mb. Oleksiewicz et al., Determination of 5 '-leader sequences from radically disparate strains of porcine reproductive and respiratory syndrome virus reveals the presence ofhighly conserved sequence motifs, ARCH VIROL, 144(5), 1999, pp. 981-987
We determined the untranslated 5'-leader sequence for three different isola
tes of porcine reproductive and respiratory syndrome virus (PRRSV): pathoge
nic European- and American-types, as well as an American-type vaccine strai
n. 5'-leader from European- and American-type PRRSV differed in length (220
and 190 nt, respectively), and exhibited only approximately 50% nucleotide
homology. Nevertheless, highly conserved areas were identified in the lead
er of all 3 PRRSV isolates, which constitute candidate motifs for binding o
f protein(s) involved in viral replication. These comparative data provide
a priori knowledge for mutational identification of virulence determinants
in the 5' nontranslated part of the PRRSV genome.