Porcine guanylin and uroguanylin: cDNA sequences, deduced amino acid sequences, and biological activity of the chemically synthesized peptides

Citation
Hj. Magert et al., Porcine guanylin and uroguanylin: cDNA sequences, deduced amino acid sequences, and biological activity of the chemically synthesized peptides, BIOC BIOP R, 259(1), 1999, pp. 141-148
Citations number
25
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
259
Issue
1
Year of publication
1999
Pages
141 - 148
Database
ISI
SICI code
0006-291X(19990527)259:1<141:PGAUCS>2.0.ZU;2-0
Abstract
Guanylin and uroguanylin are structurally related intestinal peptide hormon es which were purified from a limited number of mammals and are capable of activating the particulate guanylate cyclase-C. Although the biological fun ctions of guanylin and uroguanylin are not yet clarified in detail, they ar e involved in the regulation of the intestinal water and electrolyte balanc e. In order to verify the general importance of this hormone system in mamm als, we cloned the corresponding cDNAs from pig. Here, we present the nucle otide sequences and the deduced amino acid sequences representing porcine g uanylin and uroguanylin. The expression patterns of the corresponding genes , as shown by Northern hybridization and RT-PCR analysis, resemble those of the human homologues. Further, we demonstrate the bioactivity of both porc ine peptide hormones by inducing the intracellular cGMP production in human T84 cells and by ion transport experiments using porcine intestinal mucosa in the Ussing chamber. (C) 1999 Academic Press.