Sh. Lee et al., Nickel(II) acetate-treated Chinese hamster ovary cells differentially express vimentin, hSNF2H homologue, and h ferritin, BIOC BIOP R, 258(3), 1999, pp. 592-595
Citations number
19
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
In probing the possible non-genotoxic molecular mechanism(s) of nickel(II)-
induced carcinogenesis, we performed a non-radioactive mRNA differential di
splay analysis for nickel(II) acetate-treated Chinese hamster ovary cells (
CHO-K1-BH4), Three out of thirty differentially expressed cDNAs had sequenc
es highly similar to known genes. Down-regulation of vimentin and a hSNF2H
homologue and up-regulation of ferritin heavy chain were confirmed by North
ern blot analysis, The expression of these mRNAs was time- and nickel(II) c
oncentration-dependent. For vimentin, the decrease in mRNA level was concur
rent with a decrease in the protein level. For ferritin, the increase in mR
NA had no effect on the protein level. Dysregulation of these gene products
signifies their involvement in the epigenetic effects of carcinogenic nick
el(II) compounds.