Human nuclear matrix contains a protein complex which can specifically recognize alpha-satellite DNA in vitro

Authors
Enukashvily, NI Lobov, IB Podgornaya, OI
Citation
Ni. Enukashvily et al., Human nuclear matrix contains a protein complex which can specifically recognize alpha-satellite DNA in vitro, BIOCHEM-MOS, 64(4), 1999, pp. 473-480
Citations number
35
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMISTRY-MOSCOW
ISSN journal
00062979 → ACNP
Volume
64
Issue
4
Year of publication
1999
Pages
473 - 480
Database
ISI
SICI code
0006-2979(199904)64:4<473:HNMCAP>2.0.ZU;2-D
Abstract
A nuclear matrix protein complex has been found which specifically binds to a-satellite DNA as revealed by gel shift assay. The complex contains three proteins-two DNA-binding proteins with molecular masses of 70 and 80 kD an d a 58-kD protein which does not bind DNA but enhances the specificity of b inding of the others to alpha-satellite DNA. The complex does not contain C ENP-A, CENP-B, CENP-C, CENP-G, or lamins A/C and B.