cDNA sequence, mRNA expression, and chromosomal localization of human carbonic anhydrase-related protein, CA-RP XI

A full-length cDNA clone of a human carbonic anhydrase-related protein, CA- RP XI encoded by CA11, was obtained and sequenced. The cDNA sequence was 14 75 bp long and predicted to encode a 328-amino acid polypeptide with a mole cular mass of 36 200 Da. The deduced amino acid sequence of CA-RP XI showed an overall similarity of 47-53% to the active site residues of other activ e CA isozymes I however, it lacked three zinc-binding histidine residues, r aising questions regarding its CA catalytic activity. Northern blot analysi s demonstrated strong expression of an approx, 1.5 kb transcript in the hum an brain, particularly in the cerebellum, cerebral cortex, and putamen. A s ingle copy of the CA11 gene was localized to the human chromosome 19q13.2-3 . These results suggest that CA-RP XI plays a general role in the human cen tral nervous system. (C) 1999 Elsevier Science B.V. All rights reserved.