Depression of metabolic rate has been recorded for virtually all major anim
al phyla in response to environmental stress. The extent of depression is u
sually measured as the ratio of the depressed metabolic rate to the normal
resting metabolic rate. Metabolic rate is sometimes only depressed to appro
x. 80% of the resting value (i.e. a depression of approx. 20% of resting);
it is more commonly 5-40 % of resting (i.e. a depression of approx. 60-95%
of resting); extreme depression is to 1 % or less of resting, or even to an
unmeasurably low metabolic rate (i.e. a depression of approx. 99-100 % of
resting). We have examined the resting and depressed metabolic rate of anim
als as a function of their body mass, corrected to a common temperature. Th
is allometric approach allows ready comparison of the absolute level of bot
h resting and depressed metabolic rate for various animals, and suggests th
ree general patterns of metabolic depression.
Firstly, metabolic depression to approx. 0.05-0.4 of rest is a common and r
emarkably consistent pattern for various non-cryptobiotic animals (e.g. mol
luscs, earthworms, crustaceans, fishes, amphibians, reptiles). This extent
of metabolic depression is typical for dormant animals with 'intrinsic' dep
ression, i.e. reduction of metabolic rate in anticipation of adverse enviro
nmental conditions but without substantial changes to their ionic or osmoti
c status, or state of body water. Some of these types of animal are able to
survive anoxia for limited periods, and their anaerobic metabolic depressi
on is also to approx. 0.05-0.4 of resting. Metabolic depression to much les
s than 0.2 of resting is apparent for some 'resting', 'over-wintering' or d
iapaused eggs of these animals, but this can be due to early developmental
arrest so that the egg has a low 'metabolic mass' of developed tissue (comp
ared to the overall mass of the egg) with no metabolic depression, rather t
han having metabolic depression of the entire cell mass. A profound decreas
e in metabolic rate occurs in hibernating (or aestivating) mammals and bird
s during torpor, e.g. to less than 0.01 of pre-torpor metabolic rate, but t
here is often no intrinsic metabolic depression in addition to that reducti
on in metabolic rate due to readjustment of thermoregulatory control and a
decrease in body temperature with a concommitant Q(10) effect. There may be
a modest intrinsic metabolic depression for some species in shallow torpor
(to approx. 0.86) and a more substantial metabolic depression for deep tor
por (approx. 0.6), but any energy saving accruing from this intrinsic depre
ssion is small compared to the substantial savings accrued from the readjus
tment of thermoregulation and the Q(10) effect.
Secondly, a more extreme pattern of metabolic depression (to < 0.05 of rest
) is evident for cryptobiotic animals. For these animals there is a profoun
d change in their internal environment - for anoxybiotic animals there is a
n absence of oxygen and for osmobiotic, anhydrobiotic or cryobiotic animals
there is an alteration of the ionic/osmotic balance or state of body water
. Some normally aerobic animals can tolerate anoxia for considerable period
s, and their duration of tolerance is inversely related to their magnitude
of metabolic depression; anaerobic metabolic rate can be less than 0.005 of
resting. The metabolic rate of anhydrobiotic animals is often so low as to
be unmeasurable, if not zero. Thus, anhydrobiosis is the ultimate strategy
for eggs or other stages of the life cycle to survive extended periods of
environmental stress.
Thirdly, a pattern of absence of metabolism when normally hydrated (as oppo
sed to anhydrobiotic or cryobiotic) is apparently unique to diapaused eggs
of the brine-shrimp (Artemia spp., an anostracan crustacean) during anoxia.
The apparent complete metabolic depression of anoxic yet hydrated cysts (a
nd extreme metabolic depression of normoxic, hypoxic, or osmobiotic, yet hy
drated cysts), is an obvious exception to the above patterns.
In searching for biochemical mechanisms for metabolic depression, it is cle
ar that there are five general characteristics at the molecular level of ce
lls which have a depressed metabolism; a decrease in pH, the presence of la
tent mRNA, a change in protein phosphorylation state, the maintenance of on
e particular energy-utilizing process (ion pumping), and the down-regulatio
n of another (protein synthesis). Oxygen sensing is now the focus of intens
e investigation and obviously plays an important role in many aspects of ce
ll biology. Recent studies show that oxygen sensing is involved in metaboli
c depression and research is now being directed towards characterising the
proteins and mechanisms that comprise this response. As more data accumulat
e, oxygen sensing as a mechanism will probably become the sixth general cha
racteristic of depressed cells.
The majority of studies on these general characteristics of metabolically d
epressed cells come From members of the most common group of animals that d
epress metabolism, those non-cryptobiotic animals that remain hydrated and
depress to 0.05-0.4 of rest.
These biochemical investigations are becoming more molecular and sophistica
ted, and directed towards defined processes, but as yet no complete mechani
sm has been delineated, The consistency of the molecular data within this g
roup of animals suggests similar metabolic strategies and mechanisms associ
ated with metabolic depression. The biochemical 'adaptations' of anhydrobio
tic organisms would seem to be related more to surviving the dramatic reduc
tion in cell water content and its physico-chemical state, than to molecula
r mechanisms for lowering metabolic rate. Metabolic depression would seem t
o be an almost inevitable consequence of their altered hydration state.
The unique case of profound metabolic depression of hydrated Artemia spp, c
ysts under a variety of conditions could reflect unique mechanisms at the m
olecular level. However, the available data are not consistent with this po
ssibility (with the exception of a uniquely large decrease in ATP concentra
tion of depressed, hydrated Artemia spp. cysts) and the question remains: h
ow do cells of anoxic and hydrated Artemia spp. differ from anoxic goldfish
or turtle cells, enabling them so much more completely to depress their me
tabolism?