Increased production of lactonizing lipase (LipL) from Pseudomonas sp strain 109 by lipids and detergents

LipL of Pseudomonas sp. strain 109 is a unique lipase capable of catalyzing macrocyclic lactone synthesis using omega-hydroxy fatty acid esters as sub strates. Several fatty acid esters were tested as inducers of LipL producti on. The addition of either soybean oil or a non-ionic detergent (Noigen HC) resulted in a 44 to 45-fold increase in extracellular LipL, and the presen ce of both resulted in a further 56-fold increase. Among the triglycerides tested, triolein was the most effective, with a 50-fold increase in LipL pr oduction. A Northern blot hybridization analysis found that the lipL transc ript increased in the presence of soybean oil or Noigen HC, indicating that the production of LipL is regulated at the transcriptional level.