Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum

A conserved region of a polygalacturonase (PG) gene from Penicillium griseo roseum was PCR amplified and used to screen a genomic library from this fun gus. The nucleotide sequence of the isolated clone (pggI) consisted of 1497 bp, including a coding region of 1251 bp. This region potentially encodes a protein of 376 amino acids, and is interrupted by two introns. Extensive homology was observed between this protein and several fungal endopolygalac turonases. DNA hybridization analyses revealed that there is a low copy num ber of pggI in the P. griseoroseum genome, probably one or two copies.