Substrate selectivity of lipases in the esterification of oleic acid, linoleic acid, linolenic acid and their all-trans-isomers and in the transesterification of cis/trans-isomers of linoleic acid methyl ester

The substrate selectivity of several microbial lipases has been examined in the esterification of oleic acid, linoleic acid, linolenic acid and their all-trans-isomers and in the alcoholysis of isomeric linoleic acid methyl e sters with n-butanol. Lipases from Candida cylindracea and Mucor miehei pre ferred fatty acids and methyl esters with a (first) cis double bond in Delt a 9-position, while Chirazyme L-5, a Candida antarctica lipase A, had a pre ference for trans-9 unsaturated substrates.