Lignin peroxidase isoforms from Streptomyces viridosporus T7A: are they a monomer based structure?

Authors
Bon, EPS Nascimento, HJ Macedo, JMB Silva, JG
Citation
Eps. Bon et al., Lignin peroxidase isoforms from Streptomyces viridosporus T7A: are they a monomer based structure?, BIOTECH TEC, 13(5), 1999, pp. 289-293
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
13
Issue
5
Year of publication
1999
Pages
289 - 293
Database
ISI
SICI code
0951-208X(199905)13:5<289:LPIFSV>2.0.ZU;2-6
Abstract
Five fractions with lignin peroxidase activity were isolated by FPLC-Mono Q from a Streptomyces viridosporus culture. F4 and F5 showed the highest spe cific activity and degree of protein homogeneity by chromatofocusing, IEF- and gradient-PAGE. The individual analysis of F4 and F5 by FPLC-Superdex 75 , showed MW that were multiples to each other (68,000; 23,000; 12,000), alt hough by SDS PAGE a sole MW of 13,500 was obtained, indicating a monomer ba sed structure. The amino-acid composition of F5 showed absence of sulfur am ino acids.