R. Toury et al., c-Myc and Mxi1 immunoreactivities in the calcifying areas of the epiphyseal-plate cartilage matrix of growing rats, BONE, 24(6), 1999, pp. 555-563
We looked for the protooncogene protein, c-Myc, its dimerization partner, M
ax, and the repressors of its transactivation activity, Mad1 and Mxi1, in t
he epiphyseal-plate cartilage matrix of growing rats by immunocytochemistry
in the electron microscope. c-Myc and Mxi1 immunoreactivities were found i
n the calcifying areas of the cartilage matrix only. There was no immunolab
eling in response to anti-Max or anti-Mad1 antibodies. Mxi1 immunoreactivit
y was mainly in the early calcifying areas, in the calcification front and
ahead of it, whereas c-Myc immunoreactivity was essentially in the incomple
tely calcified regions of the matrix. The two immunolabelings occurred main
ly over the large type II collagen fibrils of the cartilage matrix and over
the thin filaments connecting them. c-Myc and Mxi1 immunoreactivities were
rarely found along the dark cristallites, There was no immunolabeling asso
ciated with the matrix vesicles, or in their immediate surroundings. The da
ta suggest that the protooncogene proteins, c-Myc and Mxi1, could be implic
ated in the calcification involving type II collagen fibrils of the epiphys
eal-plate cartilage. The absence of Max immunoreactivity from the calcifyin
g cartilage matrix raises the question of whether there are other c-Myc- an
d Mxi1-dimerization partners. (Bone 24:555-563; 1999) (C) 1999 by Elsevier
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