Two patients with novel BCR ABL fusion transcripts (e8/a2 and e13/a2) resulting from translocation breakpoints within BCR exons

We have identified novel BCR-ABL mRNA fusions by RT-PCR in two patients wit h Philadelphia (Ph) chromosome positive leukaemia. Sequencing revealed in-f rame fusions consisting of part of BCR exon e8 spliced to ABL exon a2 in on e patient and part of BCR exon e13 spliced to ABL exon a2 in the other. The breaks within BCR exons e8 and e13 did not conform to consensus splice sit es, suggesting that the aberrant fusion mRNAs may have arisen as a result o f translocation breakpoints at these sites. This was confirmed by genomic D NA bubble PCR for the second patient. These data show that BCR-ABL transloc ation breakpoints can occasionally occur within coding exons.