Differential expression of CYP1A1, CYP1A2, CYP1B1 in human kidney tumours

The presence of mRNA of individual members of the CYP1 gene family in norma l and neoplastic kidney has been investigated by RTPCR, CYP1B1 mRNA was con sistently expressed in both normal and neoplastic kidney while CYP1A1 was p resent in the majority of normal and neoplastic whereas CYP1A2 was infreque ntly expressed. Expression of the Ah receptor and Amt which are involved in the transcriptional activation of the CYP1 genes was also studied, The Ah receptor mRNA and Amt mRNA were consistently expressed both in kidney tumou rs and normal kidney, These results indicate differential expression of ind ividual members of the CYP1 gene family in normal and neoplastic kidney and suggest that several mechanisms including the Ah receptor complex could be involved in their regulation. (C) 1999 Elsevier Science Ireland Ltd. All r ights reserved.