NMR study of a Lewis(x) pentasaccharide derivative: solution structure andinteraction with cations

Authors
Henry, B Desvaux, H Pristchepa, M Berthault, P Zhang, YM Mallet, JM Esnault, J Sinay, P
Citation
B. Henry et al., NMR study of a Lewis(x) pentasaccharide derivative: solution structure andinteraction with cations, CARBOHY RES, 315(1-2), 1999, pp. 48-62
Citations number
49
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
00086215 → ACNP
Volume
315
Issue
1-2
Year of publication
1999
Pages
48 - 62
Database
ISI
SICI code
0008-6215(19990131)315:1-2<48:NSOALP>2.0.ZU;2-X
Abstract
The structure and conformation of the synthetic pentasaccharide Gal(beta 1- 4){Fuc(alpha 1-3)}GlcNAc(beta 1-3)Gal(beta 1-4)Glc-beta OMe of the Lewis(x) family has been determined by NMR spectroscopy in dimethyl sulfoxide and m ethanol. In these solvents, the binding constants with calcium have been ev aluated as 9.5 and 29.6 M-1, respectively. Study of the interaction sites h as been achieved through the use of paramagnetic divalent cations and dista nce triangulation methods. Two regions have been found, the first one in th e vicinity of the fucose unit, the second one closer to the lactose part. ( C) 1999 Elsevier Science Ltd. All rights reserved.