Enzymatic synthesis of Kdn oligosaccharides by a bacterial alpha-(2 -> 6)-sialyltransferase

Authors
Kajihara, Y Akai, S Nakagawa, T Sato, R Ebata, T Kodama, H Sato, K
Citation
Y. Kajihara et al., Enzymatic synthesis of Kdn oligosaccharides by a bacterial alpha-(2 -> 6)-sialyltransferase, CARBOHY RES, 315(1-2), 1999, pp. 137-141
Citations number
16
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
00086215 → ACNP
Volume
315
Issue
1-2
Year of publication
1999
Pages
137 - 141
Database
ISI
SICI code
0008-6215(19990131)315:1-2<137:ESOKOB>2.0.ZU;2-1
Abstract
Synthesis of CMP-deaminoneuraminic acid (CMP-beta-D-Kdn) and its enzymatic transfer reaction using bacterial alpha-(2 --> 6)-sialyltransferase were ex amined. CMP-beta-D-Kdn was prepared from methyl 4,5,7,8,9-penta-O-acetyl-3- deoxy-D-glycero-beta-D-galacto-2-nonulopyranosonate (2) in 24% overall yiel d. Enzymatic synthesis of Kdn oligosaccharide with CMP-beta-D-Kdn (10.2 mu mol), methyl beta-D-lactosaminide (7, 8.1 mu mol) and purified sialyltransf erase (80 munits) afforded Kdn-alpha-(2 --> 6)-Gal-beta-(1 --> 4)-GlcNAc-be ta-1-OMe in 77% yield. (C) 1999 Elsevier Science Ltd. All rights reserved.