Temperature-induced precipitation of specific DNA fragments using DNA-poly(N-isopropylacrylamide) conjugate

Authors
Umeno, D Maeda, M
Citation
D. Umeno et M. Maeda, Temperature-induced precipitation of specific DNA fragments using DNA-poly(N-isopropylacrylamide) conjugate, CHEM LETT, (5), 1999, pp. 381-382
Citations number
14
Categorie Soggetti
Chemistry
Journal title
CHEMISTRY LETTERS
ISSN journal
03667022 → ACNP
Issue
5
Year of publication
1999
Pages
381 - 382
Database
ISI
SICI code
0366-7022(199905):5<381:TPOSDF>2.0.ZU;2-S
Abstract
Soluble conjugates between double stranded DNA and poly(N-isopropylacrylami de) (polyNIPAAm) was synthesized and applied to the selective separation of DNA fragments: the target fragments were connected to the DNA-polyNIPAAm c onjugate with the aid of T4 DNA ligase, followed by temperature-induced pre cipitation. Target DNA was collected from the resultant precipitate in high purity.