The effects of dipyridamole stress test on plasma levels of soluble adhesion molecules intracellular adhesion molecule-1, vascular cell adhesion molecule-1, E-selectin and L-selectin in patients with ischemic heart disease and patients with syndrome X
T. Siminiak et al., The effects of dipyridamole stress test on plasma levels of soluble adhesion molecules intracellular adhesion molecule-1, vascular cell adhesion molecule-1, E-selectin and L-selectin in patients with ischemic heart disease and patients with syndrome X, CORON ART D, 10(4), 1999, pp. 235-240
Background Adhesion of activated leukocytes to the endothelium as a result
of myocardial ischemia/reperfusion has been shown to be involved in the dev
elopment of tissue injury Leukocyte adhesion to the endothelium occurs via
adhesion molecules expressed on the surface of both cell types. Upon cell a
ctivation these proteins may be released into the circulation and measured
in a soluble form.
Aim To verify whether the dipyridamole stress test, performed in patients w
ith ischemic heart disease (IHD) and in patients with syndrome X, modifies
plasma levels of the soluble adhesion molecules vascular cell adhesion mole
cule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), E-selectin and
L-selectin.
Methods Plasma levels of the soluble endothelial adhesion molecules ICAM-1,
VCAM-1 and E-selectin, as well as of the soluble leukocyte adhesion molecul
e L-selectin, were measured in venous blood samples taken before and 7 min
after administration of dipyridamole in patients with IHD, patients with sy
ndrome X and healthy individuals. Myocardial perfusion was evaluated using
single photon emission tomography. The plasma levels of soluble VCAM-1, ICA
M-1, E-selectin and L-selectin were all measured using enzyme-linked immuno
sorbent assays.
Results After infusion of dipyridamole, plasma levels of ICAM-1 increased s
ignificantly in patients with IHD, whereas they remained unchanged in patie
nts with syndrome X and in the control group. In patients with IHD, the ini
tial plasma levels of VCAM-1, E-selectin and L-selectin, before administrat
ion of dipyridamole, were higher than those observed in patients with syndr
ome X and than those in the control group. Plasma levels of soluble VCAM-1,
E-selectin and L-selectin decreased significantly in patients with IHD fol
lowing the dipyridamole stress test, whereas they remained unchanged in pat
ients with syndrome X, and in the control group.
Conclusion In patients with IHD, administration of dipyridamole induces myo
cardial ischemia resulting in modification of plasma levels of the soluble
adhesion molecules. Coronary Artery Di's 10:235-240 (C) 1999 Lippincott Wil
liams & Wilkins.