Are lipid phase transitions responsible for chilling damage in human platelets?

In previous studies we have proposed that the well-known chilling-induced a ctivation of human blood platelets can be ascribed at least in part to a th ermotropic phase transition in membrane lipids. The evidence that this is t he case is reviewed and amplified in this review, followed by an examinatio n of the available physical data concerning phase transitions in lipid mixt ures that mimic the mixture found in platelet membranes. Assuming complete mixing at all temperatures and equal contributions of the members of the mi xture to the phase transition, the lipid mixture found in platelets should give values for T-m ranging from about 1 degrees C to about 16 degrees C, d epending on the isomers present in the mixture. (The former value is not in agreement with the observed T-m, but the lader is in excellent agreement.) However, examination of the phase diagram for a binary pair of lipids foun d in platelet membranes shows that ideal mixing almost certainly does not o ccur; instead of a linear phase dia,sram, a convex one was obtained. This s hape for the phase diagram, which would displace T-m to an unexpectedly ele vated temperature, is in agreement with previously published phase diagrams for mixtures of this type. The prediction, based on thermodynamic properti es of lipids found in the platelets, is that T-m will be displaced upward i n more complex mixtures of the composition found in platelets, a prediction that requires experimental testing. (C) 1999 Academic Press.