Polymorphic forms of human ADP-ribosyltransferase-1 - Differences in theircatalytic activities revealed by labeling of membrane-associated substrates

Full length cDNA encoding ADP-ribosyltransferase-1 (ART1) was generated fro m human skeletal muscle. A single base variation from the published sequenc e was observed (C770-->T), and was established as a polymorphism by the scr eening of a population of 50 Caucasians. The base variation predicted a non conservative substitution of Leu for Pro at codon 257. Cell lines with stab le and doxycycline-inducible expression of the two polymorphic forms of ART 1 were generated from Chinese hamster V79 cells, and exploited in studies t o compare the activities of ART1-Pro257 and ART1-Leu257. The results reveal ed no differences in the capacity of phosphoinositide-specific phospholipas e C to cleave the two ART1 isoforms from the plasma membrane. Furthermore, the capacities of ART1-Pro257 and ART1-Leu257 to ADP-ribosylate agmatine or fibroblast growth factor-2 were similar. Differences in the catalytic acti vities of ART1-Pro257 and ART1-Leu257 were however, identified when measure ments were made of their capacities to ADP-ribosylate membrane-associated p roteins on the surface of V79 cells. Protein(s) of molecular mass 80-110 kD a were more extensively ADP-ribosylated by ART1-Pro257 than ART1-Leu257, in accordance with the V-max (59.5 +/- 5.5 and 37.0 +/- 3.0) and K-m values ( 12.5 +/- 4.5 and 5.0 +/- 1.9) for ART1-Pro257 and ART1-Leu257, respectively .