Mechanism of metabolite transfer in coupled two-enzyme reactions involvingaldolase

Authors
Pettersson, H Pettersson, G
Citation
H. Pettersson et G. Pettersson, Mechanism of metabolite transfer in coupled two-enzyme reactions involvingaldolase, EUR J BIOCH, 262(2), 1999, pp. 371-376
Citations number
16
Categorie Soggetti
Biochemistry & Biophysics
Journal title
EUROPEAN JOURNAL OF BIOCHEMISTRY
ISSN journal
00142956 → ACNP
Volume
262
Issue
2
Year of publication
1999
Pages
371 - 376
Database
ISI
SICI code
0014-2956(199906)262:2<371:MOMTIC>2.0.ZU;2-I
Abstract
Transient-state kinetic experiments and analyses have been performed to exa mine the validity of hitherto unchallenged evidence proposed to be indicati ve of a channelled transfer of triose phosphates from aldolase to glycerald ehyde-3-phosphate dehydrogenase and glycerol-3-phosphate dehydrogenase. The results lend no support to such proposals, but show that the kinetic behav iour of the examined aldolase-dehydrogenase reactions is fully consistent w ith a free-diffusion mechanism of metabolite transfer.